We offer a comprehensive ribosome profiling service – from initial library preparation to final data-analysis & interpretation. This encompasses the use of a combination of transcriptomics (RNA-Seq) and translatomic (Ribo-Seq) services and leverages the unsurpassed experience and expertise of our world-class team of molecular biologists and bioinformaticians.
We thus have the toolbox and expertise to provide our clients with a full end-to-end service or to provide individual components from this toolbox.
Experimental Design & Data Generation
Experimental Design & Data Generation
Generation of RNA-Seq and Ribo-Seq libraries
Standardized and customized bioinformatic analysis of Ribo-Seq and RNA-Seq data
Computational Data Analysis
Standard, customized, and comprehensive bioinformatic analysis
Providing further bespoke analysis and actionable insights and recommendations
Ancillary Services
Follow-up experiments, western blots, qPCR, luciferase reporter assays
The following are examples of typical problem statements we receive:
Carry out run-off experiments to be able to infer rates of protein synthesis from Ribo-seq data
Investigate whether chemical exposure induces toxicological effects on the translatome
Identify translated open reading frames in a poorly annotated cell line
Detect and quantify open reading frames at the individual isoform level
Determine whether a compound of interest induces ribosomal stalling on select transcripts
Discover transcripts whose translation is regulated open reading frames upstream of their protein coding region in response to a compound of interest
Estimate codon elongation rates in response to selective amino acid deprivation
Pinpoint cases of stop codon readthrough by interrogating ribosome occupancy in 3’ trailer regions