Ribomaps | Services
21486
page-template-default,page,page-id-21486,ajax_updown_fade,page_not_loaded,,select-child-theme-ver-1.0.0,select-theme-ver-4.5,menu-animation-underline,wpb-js-composer js-comp-ver-5.5.2,vc_responsive
 
Mapping the route to drug success

Services

We offer a comprehensive ribosome profiling service – from initial library preparation to final data-analysis & interpretation. This encompasses the use of a combination of transcriptomics (RNA-Seq) and translatomic (Ribo-Seq) services and leverages the unsurpassed experience and expertise of our world-class team of molecular biologists and bioinformaticians.

 

 

We thus have the toolbox and expertise to provide our clients with a full end-to-end service or to provide individual components from this toolbox.

team_image

Experimental Design & Data Generation

Experimental Design & Data Generation

Generation of RNA-Seq and Ribo-Seq libraries

team_image

Standardized and customized bioinformatic analysis of Ribo-Seq and RNA-Seq data

Computational Data Analysis

Standard, customized, and comprehensive bioinformatic analysis

team_image

Providing further bespoke analysis and actionable insights and recommendations

Ancillary Services

Follow-up experiments, western blots, qPCR, luciferase reporter assays

The following are examples of typical problem statements we receive:

 

  • Carry out run-off experiments to be able to infer rates of protein synthesis from Ribo-seq data
  • Investigate whether chemical exposure induces toxicological effects on the translatome
  • Identify translated open reading frames in a poorly annotated cell line
  • Detect and quantify open reading frames at the individual isoform level
  • Determine whether a compound of interest induces ribosomal stalling on select transcripts
  • Discover transcripts whose translation is regulated open reading frames upstream of their protein coding region in response to a compound of interest
  • Estimate codon elongation rates in response to selective amino acid deprivation
  • Pinpoint cases of stop codon readthrough by interrogating ribosome occupancy in 3’ trailer regions